anonymous 16352 Menthol-DCMU bleaching and re-inoculation of Symbiodiniaceae in the large benthic foraminifera Sorites orbiculus Timme, Lukas Theodor; Henschen, Helena; Puerto Rueda, Diana N; Manda, Sneha; Parkinson, John Everett; Wild, Christian; Abramovich, Sigal; Morard, Raphael; Schmidt, Christiane (2026): Menthol-DCMU bleaching and re-inoculation of Symbiodiniaceae in the large benthic foraminifera Sorites orbiculus [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.984033 Sorites orbiculus specimens were collected on 26 July 2023 from the Gulf of Aqaba at the Interuniversity Institute for Marine Sciences in Eilat. In a controlled laboratory setting at the Leibniz Institute for Tropical Marine Research MAREE facility, these specimens underwent a bleaching process using 0.19 mmol L-1 menthol and 5 µmol L-1 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea (DCMU) over 27 days. Post-bleaching, the specimens were re-inoculated for 19 days with the Symbiodiniaceae strains Symbiodinium microadriaticum (strains CCMP2467 and KB8) and Fugacium kawagutii (strain F2) at a concentration of 1 × 10⁵ cells mL⁻¹. Subsequently, the specimens were cultivated for an additional 37 days to assess the stability of the newly established symbiotic associations. Throughout the experiment, physiological parameters were monitored using confocal laser scanning microscopy, pulse-amplitude modulated fluorometry, standard microscopy, motility assessments, and real-time quantitative polymerase chain reaction to confirm symbiont identity. 16360 https://doi.org/10.1594/PANGAEA.984033 2026-02-02T11:41:22+0000 2026 21965 21966 21967 21968 21969 21970 21971 21972 21973 21974 21509 21975 21976 14124 21977 21978 21508 21979 21984 21983 21990 21981 21989 21986 21980 21988 21985 21982 21987